Recombinant DNA

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Recombinant DNA is one kind of new DNA that is formed by a Biotechnological process by inserting a piece of DNA part from one DNA into another DNA. Where DNA forms naturally there Recombinant DNA forms artificially. It is an artificial application of Genetic Enginering.

Recombinant DNA is also called rDNA. Another name of rDNA is mythecal chimera. In rDNA the sequence of gene is palindromic.

Once sc ientist realised that it is possible to form new structured DNA with a combination of two or more DNA strands. Because, in all DNA have same chemical elements, but different in their sequence and arrangement.

How a recombinant DNA is produces?

There need a host cell to form rDNA. In most case, in laboratory VIVO DNA replication process is allowed to form rDNA.

Cloning vector is a specific DNA strand that is allowed to repklicate in a host living cell. The two strand of DNA may be in different type. Human DNA strand can be replicate with the fungal DNA strand. Any fungal DNA strand may be replicate into bacterial DNA strand.

Steps of producing recombinant DNA

There exactly follows the following stepks for recombinant DNA technology

Selecting a host living cell and cloning vector, preparation of vector DNA, The DNA that will be cloned is prepared, Preparation of rDNA, Hosting of prepared rDNA, Selecting an appropriate organism containing rDNA






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